KMID : 0191120220370290227
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Journal of Korean Medical Science 2022 Volume.37 No. 29 p.227 ~ p.227
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Objective Interpretation of the Rapid Urease Test for Helicobacter pylori Infection Using Colorimetry
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Pih Gyu-Young
Noh Jin-Hee Ahn Ji-Yong Han Gill-Sang Jung Hyun-Suk Jung Hwoon-Yong Kim Jung-Mogg
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Abstract
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Background: The rapid urease test (RUT) is a major diagnostic tool for detecting Helicobacter pylori infection. This study aimed to establish an objective method for measuring the color changes in the RUT kit to improve the test¡¯s diagnostic accuracy.
Methods: A UV-visible spectrophotometer was selected as the colorimeter; experiments were conducted in three stages to objectively identify the color changes in the RUT kit.
Results: First, the urea broth solution showed an identifiable color change from yellow to red as the pH increased by 0.2. The largest transmittance difference detected using the UV-visible spectrophotometer was observed at a 590-nm wavelength. Second, the commercialized RUT kit also showed a gradual color change according to the pH change detected using the UV-visible spectrophotometer. Third, 13 cases of negative RUT results with a biopsy specimen and 16 of positive RUT results were collected. The transmittance detected using the UV-visible spectrophotometer showed a clear division between the positive and negative RUT groups; the largest difference was observed at a 559-nm wavelength. The lowest transmittance in the negative RUT group was 64, while the highest in the positive RUT group was 56, at the 559-nm wavelength. The UV-visible spectrophotometry reading showed a consistency of 92.7% compared with that of manual reading.
Conclusion: A transmittance of 60 at a 559-nm wavelength detected using UV-visible spectrophotometer can be used as a cutoff value for interpreting RUT results; this will help develop an automatic RUT kit reader with a high accuracy.
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KEYWORD
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Helicobacter pylori, Rapid Urease Test, Diagnosis, Spectrophotometer
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